Annegret Potthoff and Wolfgang Unger (2019), Report on SOP for dispersing nanomaterials in water and report on round robin test.



Potthoff, Annegret (Fraunhofer IKTS)

Unger, Wolfgang (BAM)

Report on SOP for dispersing nanomaterials in water and Report on round robin test. 

Dresden, 2019, 24 P. 


This report describes a standard operation procedure for dispersion of nanomaterials in liquids prior to toxicological or ecotoxicological testing. Main factors, who determine the state of agglomeration and aggregation of ENMs after dispersion are specific energy input, particle concentration and fluid composition. The method was validated in two round robin tests, where two typical nanomaterials (a nanopowder and a nanodispersion) were investigated. One main result arising out of the data comparison was that only those participants, who were able to follow the instructions in the SOPs completely, received similar results regarding particle size and zeta potential. The SOP is easy to adapt for other types of nanomaterials. The results provide the standardization process and were presented at DIN working group. 

Open Access Publication 

Key words: nanoparticle; particle size; zeta potential; round-robin test


European Commission EC 


Name of project:

Development of reference methods for hazardidentification, risk assessment and LCA of engineered nanomaterials

Acronym: NanoValid 

Theme 4: Nanosciences, Nanotechnologies, Materials andnew Production Technologies and Theme 6: Environment (including Climate Change)


Contributors (co-authors with participant short name): NICPB; INMETRO; EAWAG; Nanologica; UoB; IMT; Veneto; BAM


The research leading to these results has received funding from the European Community's Seventh Framework Programme (FP7/2007-2013) under Grant Agreement n° 263147

Language: Englisch (DOI:

“Risk Assessment and Risk Management of Nano-Biomaterials in Medical Applications”

16–20 April 2018
Venice, Italy


As a follow-up of the first NanoDefine industry-focused workshop in 2016, a second one was arranged in the final year of the project on 24 October at DECHEMA Frankfurt, together with the German VCI. Main purpose of this event was the practical demonstration of key measurement techniques developed to industrial end-users, regulatory bodies, enforcement labs and to the wider community. This final NanoDefine dissemination event has attracted about 80 representatives from all over Europe (including one participant from Japan!) and from a wide variety of stakeholders, including material manufacturers, especially from the paint, pigment and fillers industry.


NanoDefine is approaching the end of the project (31 October 2017). By assessing a wide spectrum of test materials from more simple monotype to complex poly-disperse matrices with different shapes, structures and compositions, including real-world materials, such as industrial or consumer products, the performance of existing key measurement techniques (MTs) was critically evaluated, improved and further developed, and validated.


NanoDefine has engaged in the EuroNanoForum 2017 on 21-23 June 2017 in Valletta, Malta, a biannual international conference supposed to be one of the most relevant European forum for scientists, industrialists and policy makers focusing on various nanotechnology fields and associated industrial sectors (


On 20 June 2017, the day before the ENF2017, experts of the NanoDefine team were actively contributing to the 2nd CEN Open Meeting “European Conference on Standardization for Nanotechnologies and Nanomaterials” held also in Valletta, Malta (, and organized by CEN/TC 352 “Nanotechnologies” in cooperation with CEN-CENELEC Management Centre, the EU Commission and EFTA.


“Measurement and classification of nanomaterials according to the EU definition”

24 October 2017 - Frankfurt am Main - Germany


Here comes the 2nd NanoDefine Bulletin highlighting the most recent results produced by the project since spring 2016 and their practical potential for end-users, such as material and instrument manufacturers, to match the analytical challenges triggered by the EC Recommendation for a Nano-definition (2011/696/EU). Main objective of the EU FP7 NanoDefine project is to develop a methodology and measurement strategy to support practitioners in industries, regulatory bodies and enforcement laboratories to reliably implement the EC Nano-definition.


Join the Final NanoDefine Outreach Event on “Classification of Materials according to the EU Nano-Definition” on the 19-20 September 2017 at “Leopold” Hotel in Brussels (Rue du Luxembourg 35, 1050 Bruxelles) ( This 2-days final meeting will give you a unique opportunity to learn more about the outcome of the NanoDefine project and possible practical implications, and how to meet future regulatory needs associated with the practical implementation of the EU recommendation on the Definition of Nanomaterial (2011/696/EU).


The concept of the European FP7 project NanoDefine is: Development of an integrated approach based on validated and standardized methods to support the implementation of the EC recommendation of the for a definition of nanomaterial. The major outcome of the project is the establishment of an integrated tiered approach based on validated rapid screening methods (tier 1) and validated confirmatory methods (tier 2). With the developed NanoDefiner eTool and user manual, end-users will be guided to implement the developed methodology.